Sequence comparison and secondary structure analysis of the N-terminal region of NDUFV2. (a) Multiple sequence alignment of NDUFV2 proteins from different species. Sequence alignment was generated by EMBL-EBI Clustal W2  and displayed by BOXSHADE server . The abbreviations used are: H. sapiens, Homo sapiens NDUFV2 (UniProt: P19404); B. Taurus, Bos taurus 24 kDa (UniProt: P04394); N. crassa, Neurospora crassa NUO-24 (UniProt: P40915); Y. lipolytica, Yarrowia lipolytica NUHM (UniProt: Q9UUT9); P. denitrificans, Paracoccus denitrificans NQO2 (UniProt: P29914); T. thermophilus, Thermus thermophilus Nqo2 (UniProt: Q56221); E. coli, Escherichia coli strain K12 NuoE (UniProt: P0AFD1). Residues identical to the consensus are highlighted in reversed-out lettering on a black background; residues not identical but similar to the consensus are shown on a grey-shaded background. (b) The secondary structure prediction of wild-type and NDUFV2 IVS2+5_+8delGTAA disease mutant. Secondary structure of the N-terminal region of NDUFV2 was predicted by the PSIPRED server . H, α-helix; C, coil; E, strand.