Real time PCR amplification of GAD mRNA form the cerebellum (A) and brain stem (B) of control and experimental neonatal rats. The ΔΔCT method of relative quantification was used to determine the fold change in expression. The relative ratios of mRNA levels were calculated using the ∆∆CT method normalized with β-actin. CT value as the internal control and Control CT value as the caliberator. PCR analyses were conducted in the cerebellum (A) and brain stem (B) with gene-specific primers and fluorescently labeled Taq probe GAD1 (Rn 00690304_g1).