Figure 1

UC-MSC cells showed a fibroblast-like morphology before conditioned culturing (AC); the induced cells change their morphology with time. In cells treated with CMCM or CMCM+S1P, HUMSCs displayed a cardiomyocyte-like morphology such as myotube-like shape between 5-7 days (D, E, G, H); At around 10 days, the cells became elongated and lined up in CMCM and CMCM+S1P groups (J, K), and the alignment of the cells appeared in an ordered perpendicular terrace-pattern, like intercalated disc in CMCM+S1P groups. (K). But the cells had no similar change in S1P+DMEM groups (F, I), and the alignment looked random. (L)