Figure 3
From: Stanniocalcin-1 promotes tumor angiogenesis through up-regulation of VEGF in gastric cancer cells
Effects of STC-1 and VEGF on HUVEC cell migration. (A) Effects of STC-1 on HUVEC migration. HUVEC were seeded in triplicate on inserts, and incubated for 12 h with different conditioned supernatants. (B) Effects of VEGF on HUVEC migration. HUVEC were seeded in triplicate on inserts, and incubated for 12 h with tumor supernatants incubated with 2 μg/mL VEGF monoclonal antibody (Bioactive). (C) The number of migration cells was quantified under a microscope at ×100 magnification. All histogram was carried out on multiple sections and the results are representative of three independent experiments. (D) Effect of isotype antibody on cell migration. IS: isotype antibody; V:VEGF neutralizing antibody; BGC/STC+IS: BGC/STC cell supernatants added with isotype antibody; BGC/STC+V: BGC/STC cell supernatants added with VEGF neutralizing antibody.