Structural and functional characterization of human apolipoprotein E 72-166 peptides in both aqueous and lipid environments

Table 2 Global discrete species analysis of apoE-(72-166) with different environments^a

In^b	ApoE2-(72-166)			ApoE3-(72-166)			ApoE4-(72-166)
In^b	S^c(Svedberg)	M^d(kDa)	Local C of SV and SE (A₂₈₀)^e	S^c(Svedberg)	M^d(kDa)	Local C of SV and SE (A₂₈₀)^e	S^c(Svedberg)	M^d(kDa)	Local C of SV and SE (A₂₈₀)^e
PBS	2.2	19	0.07, 0.10	2.1	18	0.07, 0	2.7	24	0.02, 0
	5.3	67	0.17, 0	4.8	68	0.16, 0.04	4.7	51	0.18, 0.05
	7.8	186	0.05, 0.11	7.7	251	0.03, 0.06	8.6	210	0.05, 0.12
5 mM DHPC	2.3	25	0.07, 0.08	2.5	21	0.08, 0.08	2.2	22	0.01, 0
	4.9	49	0.18, 0	4.9	52	0.15, 0	4.8	51	0.17, 0.07
	8.2	190	0.04, 0.09	8.4	232	0.03, 0.06	8.5	217	0.04, 0.19
50 mM DHPC	1.3	19	0.35, 0.13	1.2	20	0.34, 0	1.1	19	0.29, 0
	4.3	71	0.01, 0.15	3.1	40	0.02, 0.15	3.8	45	0.03, 0.14
100 mM DHPC	1.2	30	0.31, 0	1.0	19	0.32, 0.08	0.8	20	0.27, 0.04
	3.3	49	0.05, 0.17	2.3	50	.0, 0.10	3.0	58	0.01, 0.11

^a The SV and SE experiments of apoE-(72-166) were combined and fitted to the global discrete model by SEDPHAT [33]. The best-fit local root mean square errors of SE were from 0.0041 to 0.0118 and those of SV were from 0.0054 to 0.0088.
^b The partial specific volume was set by 0.73 in PBS and 5 mM DHPC and by 0.86 in 50 and 100 mM DHPC (see materials and methods for detail).
^{c, d, e} Best-fit calculated sedimentation coefficients (s), molar mass (M), and local concentrations (C) of different species were shown. The local concentration of SV was from the discrete distribution of SV and that of SE was from the SE experiments. The concentration units were signal units (A₂₈₀).

ISSN: 1423-0127