Figure 4

Inhibition of trypsin-induced cyclooxidase-2 (COX-2) expression by proteinase-activated receptor-2-inhibiting peptide (PAR2-IP) in human synoviosarcoma cells. Human synoviosarcoma SW982 cells were treated with the PAR2-IP, IL-1β, and/or trypsin in serum-free L15 medium. Expressions of COX-2 and MMP-1 were analyzed by western blotting, and relative levels were calculated by normalizing the band densities to that of GAPDH and setting the zero controls as 100%. (A) Cells were treated with IL-1β at 5 ng/ml or PAR-2 AP at different concentrations for 24 hours. (B) Cells were pretreated with PAR2-AP at different concentrations for 30 minutes and then incubated with trypsin at 30 nM for 6 hours.