Figure 2

The cDNA sequence of the truncated form of MsrA with the predicted open reading frame. Thin lines indicate exon-exon junctions. A. The thick line points to the junction of exon 4 and 6 skipping exon 5 in the truncated form but is included in the long form of MsrA. Shaded sequences are the two primers used to amplify the whole cDNA. Sequences underlined show the primer pairs for real time PCR to specifically amplify the truncated form with the forward primer designed on the junction of exon 4 and 6. B. The truncated form contains 148 amino acids with a mitochondrial signal peptide at the N-terminus and a GCFWG motif but no c-terminal cysteines. C. Schematic illustration of exon compositions for the long form and truncated MsrA cDNA. Arrows point to the translation stop sites.