The effects of RSV on superoxide anion production, lipid peroxidation, protein carbonyl level, MnSOD and CuZnSOD protein expressions in the renal tissues of STZ-DM rats. (A) Superoxide anion content was measured by lucigenin-enhanced chemiluminescence. (B) Lipid peroxidation was determined using the modified thiobarbituric acid reactive substances method. (C) Protein carbonyl level was evaluated by 2,4-dinitrophenylhydrazine method. (D) Equal amounts of proteins were resolved on 10 and 15% SDS-PAGE and blotted with MnSOD and CuZnSOD antibodies, respectively. The blots were shown at the top and the quantified ratios were shown at the bottom. Results were expressed as means ± standard error (n = 5 per group). *: P < 0.05 vs. control, †: P < 0.05 vs. STZ-DM, ‡: P < 0.05 vs. DM-R0.1, RLU: relative light unit, LPO: lipid peroxidation, PCL: protein carbonyl level, SOD: superoxide dismutase, CON: non-diabetic control, STZ-DM: streptozotocin-induced diabetes, DM-R0.1: DM treated with RSV (0.1 mg/kg/day) for 7 days, DM-R1: DM treated with RSV (1 mg/kg/day) for 7 days.