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Table 5 Real Time PCR amplification of bax, ubiquitin carboxy-terminal hydrolase, α-Synuclien and CREB mRNA in the Cerebral cortex of experimental rats

From: Enhanced glutamate, IP3 and cAMP activity in the cerebral cortex of Unilateral 6-hydroxydopamine induced Parkinson's rats: Effect of 5-HT, GABA and bone marrow cell supplementation

Animal Status

Log RQ

 

bax

ubiquitin carboxy-terminal hydrolase

α-Synuclien

CREB

Control

0

0

0

0

6-OHDA

1.96 ± 0.18a

0.99 ± 0.06 a

-3.12 ± 0.31a

-2.91 ± 0.22a

6-OHDA +5HT

1.02 ± 0.19 b, f

0.51 ± 0.05 b, f

-1.41 ± 0.29b,e

-1.32 ± 0.13b, f

6-OHDA +GABA

1.06 ± 0.11 b, f

0.50 ± 0.07 b, f

-1.55 ± 0.26b,e

-1.43 ± 0.12b, f

6-OHDA +BMC

1.79 ± 0.10a

0.98 ± 0.04 a

-2.99 ± 0.24a

-2.65 ± 0.21a

6-OHDA +5HT + BMC

0.64 ± 0.10 c, e

0.23 ± 0.06c, e

0.12 ± 0.09d

-0.56 ± 0.08c, e

6-OHDA + GABA + BMC

0.61 ± 0.07c,e

0.26 ± 0.04c, e

0.13 ± 0.12d

-0.59 ± 0.09c, e

6-OHDA +5HT + GABA+ BMC

0.29 ± 0.06d

0.11 ± 0.02d

0.41 ± 0.13d

0.09 ± 0.03d

  1. Values are Mean ± S.E.M of 4-6 separate experiments. Each group consist 6-8 rats.
  2. a p < 0.001, b p < 0.01, c P < 0.05 when compared to Control,
  3. d p < 0.001, e p < 0.01, f P < 0.05 when compared to 6-OHDA group.
  4. C - Control, 6-OHDA - 6-OHDA infused, 6-OHDA +5-HT - 6-OHDA infused treated with Serotonin, 6-OHDA +GABA - 6-OHDA infused treated with GABA, 6-OHDA +BMC- 6-OHDA infused treated with BMC, 6-OHDA +5-HT+BMC- 6-OHDA infused treated with Serotonin and BMC, 6-OHDA + GABA +BMC- 6-OHDA infused treated with GABA and BMC, 6-OHDA +5-HT + GABA+ BMC- 6-OHDA infused treated with Serotonin, GABA and BMC. The relative ratios of mRNA levels were calculated using the ΔΔCT method normalized with β-actin CT value as the internal control and Control CT-value as the calibrator.