Evaluation of the expressions of alcoholic fatty liver (AFL) biomarkers using western blotting. (A) Confirmation of C-reactive protein (CRP) and haptoglobin (Hp) expression by Western blotting. AAT was used as an indicator represented as a positive inflammatory protein for gastrointestinal inflammation (B) The semi-quantification of the results of Western blotting. CRP increased in the serum of AFL rats compared to normal, NAFL and liver fibrosis ones, but decreased in TAA-induced liver fibrosis. (C) Hp increased in rats suffering from NAFL, but decreased in liver fibrosis compared to the controls. (D) AAT was not affected among the rats. Three individual samples of healthy controls, NAFL, and liver fibrosis, and five samples of AFL were examined by using Western blotting. N, normal controls; AFL, alcoholic fatty liver; NAFL, non-alcoholic fatty liver; CRP, C-reactive protein; Hp, haptoglobin; AAT, alph1 antitrypsin. *p < 0.05, compared to healthy controls; #p < 0.05 as compared to the other groups.