Immunohistochemistry staining for murine Purkinje cells in cerebellum. (A) Whole brain sections of wild-type mouse, SCA2 Tg mouse as control, and SCA2 transgenic mouse which received intravenous and intracranial human mesenchymal stem cells transplantation (4 μm) were processed by immunohistochemistry of calbindin D28K for Purkinje cells. Photographs were taken from the view of 100-folds microscopy and the scale bar was 40 μm. (B) Quantitative counting of calbindin D28K+ cells in cerebellum were compared to those of Control-Wt. Statistical analysis was performed by one-way ANOVA. (* p < 0.05; ** p < 0.001).