Figure 1
From: Involvement of mTOR signaling in sphingosylphosphorylcholine-induced hypopigmentation effects
Effects of SPC on melanogenesis in Mel-Ab cells. (A) Cells were treated with SPC at various concentrations (0-20 μM) for 24 h and cell viability was determined using a crystal violet assay. (B) Cells were incubated with 0-10 μM SPC for 4 d, and phase contrast microscopy photographs were obtained using a digital video camera. Melanin contents (C) and tyrosine activity (D) were analyzed as described in 'Materials and Methods'. Data represent the mean ± SD of triplicate assays expressed as percentages of the control. ** P < 0.01 compared to the untreated control.