Soluble Notch ligands activated Notch signaling and amplified LPS-induced inflammatory responses. (A) Soluble Jagged1 (sJag1; 1 μg/ml) augmented LPS-induced Hes5 and Hey2 mRNA expression in macrophages. sDll1 (B) and sDll4 (C) also had a similar effect. (D) sJag1, sDll1, and sDll4, especially sDll1 (all 1 μg/ml), significantly enhanced LPS-induced NICD formation. (), fold change as compared to basal level. sDll1 significantly amplified LPS-induced IL-1β (E) and IL-6 (F) secretion in macrophages. *, P < 0.05.