Figure 7

Pre-treatment with ceftriaxone induced the GLT1 protein expression in cerebral cortical neuron. Immunohistochemistry staining of GLT1 (DAB: brown) and counter-staining with Nissl stain (blue) in cerebral cortex of P7 rat. A: pre-treatment with 3 dosages of saline, B: pre-treatment with 3 dosages of 200 mg/kg ceftriaxone. Arrow indicated neuronal expression of GLT1 protein. Scale bar = 20 μm. C: percentage of area of immunohistochemical staining for GLT1 under 400X light microscope in saline and ceftriaxone treated groups. (n = 3 in saline and n = 4 in ceftriaxone group, *: p < 0.05.) Saline group, animals received hypoxic-ischemic procedures and given saline injection