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Table 4 qRT-PCR confirming miR-1 down regulates genes in different cell lines

From: MicroRNA-1 induces apoptosis by targeting prothymosin alpha in nasopharyngeal carcinoma cells

Symbol\cell NPC-TW01 NPC-TW06 HEK-293T Cal-27 HeLa HepG2 KYSE30 SAS SW620
C5 0.16 0.71 0.19 0.44 0.21 0.24 0.32 0.11 1.53
CARD8 0.44 0.94 0.17 0.63 1.04 0.31 0.04 0.58 0.71
FAIM 0.22 0.88 0.21 0.62 0.80 0.29 0.44 0.21 1.32
CRIN2A 1.05 0.19 0.09 1.36 0.43 0.24 0.80 1.51 0.31
PTMA 0.29 0.28 0.28 0.26 0.45 0.45 0.42 0.11 0.62
TP63 0.69 1.12 0.07 2.45 0.92 0.27 1.00 0.88 0.43
  1. Nine cell lines were transfected with or without miR-1; total RNA was isolated from these cells after 24 hours of transfection. mRNA from the six candidate genes were quantitated by qRT-PCR, normalized to GAPDH mRNA, and compared to levels in the untransfected samples. PTMA mRNA is down regulated in all nine miR-1 transfected cell lines.