Figure 4

Histograms of relative proportions of neuron area in wild-type and dt/dt mice. Neuron areas in sympathetic ganglia and in ciliary ganglia of wild-type (n = 5) and dt/dt (n = 4) mice were determined (A and B). Neuron areas were sorted into groups at 50 μm² intervals and the percentage distributions of neuron sizes were divided into classes of the same size range. The greatest proportion of sympathetic neuron ranged between 401 and 450 μm² in wild-type mice, whereas the greatest proportion ranged between 301 and 350 μm² in dt/dt mice (A). Photomicrographs revealed that the reduced size of sympathetic ganglion and neuron in dt/dt mice compared with those in wild-type mice (a1-a4). Moreover, the greatest proportion of neuron ranged between 351 and 400 μm² in ciliary ganglia of wild-type mice, whereas the greatest proportion ranged between 301 and 350 μm² in dt/dt mice (B). Photomicrographs reappeared that the smaller size of ciliary ganglia and neurons in dt/dt mice compared with those in wild-type mice (b1-b4). Scale bars = 100 μm.