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Figure 6 | Journal of Biomedical Science

Figure 6

From: Mislocalization of the exitatory amino-acid transporters (EAATs) in human astrocytoma and non-astrocytoma cancer cells: effect of the cell confluence

Figure 6

Cell fractionation of sub-confluent and confluent human cancer cells. Astrocytoma brain cancer (STTG-1), adenocarcinoma (Caco-2) and non-astrocytoma brain cancer (SH-SY5Y and HOG) cells were used. Sub-confluent and confluent human cancer cells were subjected to cell fractionation and western-blot analysis as explained in Materials and Methods. Nitrocellulose membranes were probed with antibodies directed against GLAST or Histone 3. Cy = cytosolic fraction, Mb = membrane fraction, Nu = nuclear fraction. Similar results were obtained with antibodies directed against GLT-1 (data not shown).

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Journal of Biomedical Science

ISSN: 1423-0127