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Table 2 Uptake and secretion of L-glutamate by human cancer cells and rat astrocytes.

From: Mislocalization of the exitatory amino-acid transporters (EAATs) in human astrocytoma and non-astrocytoma cancer cells: effect of the cell confluence

 

Glutamate uptake

Glutamate secretion

 

Sub-confluent cells

Confluent cells

Sub-confluent cells

Confluent cells

 

control

+PDC

control

+PDC

control

+Sulfa

control

+Sulfa

AGS

1.2 ± 2.7

0.7 ± 1.9

89 ± 15.2

1.9 ± 3.3

18 ± 4.7

0.9 ± 2.3

0.1 ± 0.9

1.2 ± 0.7

Caco-2

1.7 ± 1.9

0.8 ± 2.4

133 ± 19

0.8 ± 1.1

21 ± 1.8

1.1 ± 0.7

0.7 ± 1.3

1.4 ± 0.9

HeLa

6.1 ± 9.1

0.3 ± 2.2

92 ± 21

2.1 ± 0.9

24 ± 9.1

1.0 ± 0.8

0.9 ± 1.1

1.1 ± 0.8

HOG

1.7 ± 3.2

0.8 ± 0.9

86 ± 9.8

1.3 ± 2.4

19 ± 3.7

0.7 ± 1.3

0.4 ± 1.9

0.9 ± 1.2

HT29

0.8 ± 1.5

0.5 ± 2.1

121 ± 8.7

1.7 ± 3.1

27 ± 3.4

0.8 ± 1.9

0.3 ± 1.4

0.1 ± 1.9

SHSY5Y

18.4 ± 9.9

0.7 ± 2.8

84 ± 14

0.9 ± 2.3

14 ± 3.1

1.1 ± 2.4

0.1 ± 0.8

0.3 ± 2.1

STTG-1

1.6 ± 0.7

0.3 ± 1.3

2.1 ± 1.1

0.7 ± 0.9

34 ± 5.6

0.2 ± 1.2

42 ± 11

1.1 ± 0.7

Astrocytes

119 ± 12

1.5 ± 8.4

145 ± 27

0.9 ± 2.7

0.1 ± 0.3

0.0 ± 1.9

0.4 ± 1.2

0.1 ± 0.9

  1. The uptake and the secretion of L-glutamate were measured as explained in Materials and Methods. Results were expressed as nmole of L-glutamate transported per mg of cellular proteins. Inhibitors used were PDC (100 μM) and sulfasalazine (250 μM). All results were obtained from three independent experiments and were expressed as means ± standard deviation (S.D). Values in bold are statistically different from the control values with p at least < 0.05.
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Journal of Biomedical Science

ISSN: 1423-0127