Figure 3
Effect of C. neoformans on the adhesion and invasion of HBMEC in the Cav1 knockdown HBMEC. HBMEC cells were pretreated with (1) mock, (2) random oligo control, and (3) Cav-1 siRNA, before (A) the adhesion and (B) the invasion analyses. The treated samples were incubated C. neoformans strain C1186 (105 cells) for 3 hr and then the slides were prepared for immunofluorescence imaging (see Materials and Methods). The mock sample (lane 1) was designated as 100%, and the effects of siRNA were indicated by percentage over the control, respectively. Each bar represents the average of four different experiments ± SD with P < 0.01. Detailed adhesion and invasion assays are described in Materials and Methods.