Figure 4

Effect of Cav1-knockdown HBMEC on C. neoformans transmigration across the monolayer. HBMEC (10⁴ cells) were seeded on the collagen-coated transwells for four days, until transendothelial electric resistance (TEER) reached > 250 μΩ/cm². The coated cultures were treated with control oligo or Cav1 siRNA, either 10 or 20 pmoles, in the 0.5 mL upper chamber, for 2 days before the transcytosis studies. CFU were counted from the lower chamber of the transwell at 4 and 8 h after the addition of C. neoformans (n = 3). Analysis of variance shows significant decreases in transcytosis ability in the Cav1 knockdown HBMEC (P = 0.011).