Figure 4
From: Anti-inflammatory effects of spermidine in lipopolysaccharide-stimulated BV2 microglial cells
Effects of spermidine on expression of IL-6 and TNF-α mRNA in LPS-stimulated BV2 microglia. Cells were pretreated with the indicated concentrations of spermidine for 1 h before LPS treatment, and the total RNAs were isolated at 6 h after LPS treatment. The levels of IL-6 and TNF-α mRNA were determined by RT-PCR. GAPDH was used as internal control.