Figure 2

Down-regulation of PKCζ in human conventional RCC tissues by Immunohistochemical staining. (A) Tissue sections of normal parenchymal tissues (a, c) or RCC (b, d) were from the same patient. The polyclonal anti-PKCζ antibody was used to stain paraffin sections (a, b). The negative control omitted the primary antibody (c, d). The brain tissue was used as a positive control for PKCζ expression (e). The tumor grade and stage in (b) and (d): grade I and TNM stage T₂N₀M₀. Original magnification, × 100. (B) Representative immunohistochemical staining of PKCζ expression in normal parenchymal and RCC tissues. (a) and (b), negative staining (0+); (c) and (d), weakly positive staining (1+); (e) and (f), strongly positive staining (2+), The tumor grade and stage in (b): grade I and TNM stage T₁N₀M₀, in (d): grade II and TNM stage T_1bN₀M₀ and (f): grade IV and TNM stage T_3aN₁M₁ (100 × magnification).