Figure 1

Schematic representation of plasmids for production of recombinant baculoviruses bearing a variety of CHIKV E1 mutants. Expression of CHIKV 6K-E1 protein (monomeric-E1 based constructs): E1-WT, E1-A226V, E1-V178A, E1-G91D and E1-H230A contain a 6K-E1 region cDNA fragment and the indicated site for substitutions (line 1 to line 5, respectively). For expression of all of the CHIKV structural polyproteins (26S-based constructs): S-WT, S-A226V, and S-V178A contain the full-length cDNA of the CHIKV 26S subgenomic RNA (AF369024) and indicated mutations (line 6 to line 8). Abbreviations: PH = polyhedrin promoter; CE26KE1 = a polyprotein translated from CHIKV 26S RNA; EGFP = enhanced green fluorescent protein gene; Rhir = RhPV 5'-UTR IRES.