Figure 8
De-ubiquitination in RVLM also remains operational during experimental brain death. Phasic changes in spot volume of ubiquitin in the proteomic map of RVLM (A); fold changes against sham-controls of monoubiquitin detected by Western blot analysis (B) or ubb mRNA determined by real-time PCR (C) in RVLM of rats that received intravenous administration of LPS (15 mg kg-1), alone or with additional pretreatment by microinjection into bilateral RVLM of UCH-L1 inhibitor (UCH-L1i; 1 nmol). Values are mean ± SEM, n = 5–6 animals per experimental group. *P < 0.05 versus sham-control group, and +P < 0.05 versus LPS group at corresponding phase in the Scheffé multiple-range test.