Atg7 Is Downstream of Hsp27 in the Regulation of Drosophila Lifespan. (A, B, E, F) RT-PCR verifies that the transcript levels of Hsp27 and Atg7 are altered upon Gal4 induction. (C, D) RNAi knockdown of Hsp27 by hs-Gal4 and da-Gal4 both reduce Drosophila lifespan. (G) Overexpression of Atg7 by hs-Gal4 increases Drosophila lifespan. (H) Knockdown of Atg7 by hs-Gal4 decreases Drosophila lifespan. (I) Neuronal overexpression of Atg7 by appl-Gal4 enhances Drosophila lifespan. (J) Overexpression of Atg7 along with knockdown of Hsp27 by appl-Gal4 displays extended lifespan. On the other hand, simultaneous knockdown of Atg7 and overexpression of Hsp27 exhibits reduced lifespan.