Figure 4
From: Expression of Nucleophosmin/NPM1 correlates with migration and invasiveness of colon cancer cells
Exogenous expression of NPM1 enhanced colon cancer cell migration and invasive ability. A. Western blotting analysis of NPM1 expression in HT29 cells transfected with control or GFP-tagged NPM1 plasmid. NPM1 and GFP antibody were used to confirm the expression of NPM1. β-actin was used as a loading control. B. MTT assay showed that elevated NPM1 expression in human colon cancer HT29 cells increased cell proliferation. *** P < 0.0001. C. BrdU incorporation rate increased after exogenous NPM1 expression in HT29 cells. ** P < 0.001. D. PCNA expression in the nucleus increased after exogenous NPM1 expression in HT29 cells. *** P < 0.0001. E. Elevated NPM1 expression in human colon cancer HT29 cells enhanced the cell invasive ability. ** P < 0.001.