Figure 4

Western Blot Analysis for ZBD. The polyclonal antibodies, anti-propeptide (RM7-P) and anti-C-terminal peptide (RM7-C), were used to detect the final purified fraction of ZBD. Panel A left: The transblot was reacted with RM7-P anti serum which specifically recognizes the proenzyme (lane P1 and P2) and the peptide22-34 conjugated BSA (lane N); Panel A right : The transblot was reacted with RM7-C anti serum which recognizes all populations of recombinant MMP-7 and the peptide238-247 conjugated BSA (lane C), except for the peptide22-34 conjugated to BSA (lane N). Both antibodies have no cross-reactivities for the BSA carrier (laneB) or each other. Panel B: The silver staining for the high resolution SDS-PAGE shows the sample loading profile. Mw: Molecular weight standard; P1 and P2: proMMP-7; A: all populations of activated MMP-7. Z: recombinant CZBD; N: peptide22-34 conjugated BSA; C: the peptide238-247 conjugated BSA. B: BSA.