Figure 1

Phosphoinositide-3-kinase (PI3K) inhibition decreases intracellular Ca²⁺, [Ca²⁺] _i , in HL-1 cell mouse cardiomyocytes. A. Effect of LY294002 (20 μM) on oscillations of [Ca²⁺]_i in three HL-1 cells showing synchronous oscillations of [Ca²⁺]_i. B. Effect of LY294002 (20 μM) on average [Ca²⁺]_i in cells displaying oscillating and non-oscillating [Ca²⁺]_i (mean ± SEM; n = 37 cells). C. Effect of LY294002 (1 μM) on [Ca²⁺]_i oscillations in five representative HL-1 cells. Time base applies to all traces. D. Effect of LY294002 (1 μM) on average [Ca²⁺]_i in cells displaying oscillating and non-oscillating [Ca²⁺]_i (mean ± SEM; n = 5, each an average of 25 to 40 cells as shown in C).