Effect of RRM-MV treatment on the DNA of cancer cell lines. MM96L cells in (A) and COLO16 cells in (B) were incubated with 200 and 400 ng/mL of RRM-C or RRM-MV for 18 h. DNA was extracted and analyzed by electrophoresis on 1.5% agarose gel with ethidium bromide staining. DNA samples are labeled as: (M) molecular weight marker, (1) untreated cells, (2) cells treated with DMSO as positive controls, (3) cells treated with 200 ng/mL RRM-C, (4) cells treated with 200 ng/mL RRM-MV, (5) cells treated with 400 ng/mL RRM-C, and (6) RRM-MV-treated cells. In MM96L cells, RRM-MV treatment produced DNA degradation, whereas in COLO16 cells, DNA degradation was not detected even with DMSO-treated samples. Results are representative of more than three independent experiments.