Table 1 Nucleotide sequences of siRNAs and their target positions in the EV71 genome (Genbank accession no. HM003207.1)
siRNA | Chemically modified | Nucleotide sequence | Genomic position |
|---|---|---|---|
si-1 | No | 5′-CAGCAAACCACGAUCAAUATT-3′(Sense) | 115-133 |
5′-UAUUGAUCGUGGUUUGCUGTT-3′(Anti-sense) | |||
si-1OMe | 2′-OMe-modified | 5′-C AGC AAACC AC GAUC AAU ATT-3′(Sense) | 115-133 |
5′-U AUU GAUC GU GGUUU GCU GTT-3′(Anti-sense) | |||
si-1 F | 2′-F-modified | 5′-C AGC AAACC AC GAUC AAU ATT-3′(Sense) | 115-133 |
5′-U AUU GAUC GU GGUUU GCU GTT-3′(Anti-sense) | |||
si-2 | No | 5′-CAGAGCAAUUGUUUACCUATT-3′(Sense) | 648-666 |
5′-UAGGUAAACAAUUGCUCUGTT-3′(Anti-sense) | |||
si -2OMe | 2′-OMe-modified | 5′-C AGAGC AAUU GUUU ACCU ATT-3′(Sense) | 648-666 |
5′-U AGGU AAAC AAUU GCUCU GTT-3′(Anti-sense) | |||
si-2 F | 2′-F-modified | 5′-C AGAGC AAUU GUUU ACCU ATT-3′(Sense) | 648-666 |
5′-U AGGU AAAC AAUU GCUCU GTT-3′(Anti-sense) | |||
scr | No | 5′-GGAUUGACCUCUCAUAUAATT-3′(Sense) | |
5′-UUAUAUGAGAGGUCAAUCCTT-3′(Anti-sense) | |||
si-2FAM | FAM-modified | 5′-(FAM) CAGAGCAAUUGUUUACCUATT-3′(Sense) | 648-666 |
5′-UAGGUAAACAAUUGCUCUGTT-3′(Anti-sense) |