Effect of different concentrations of daidzein on activation of Src kinase and ERK. DRG neurons were treated with 0, 30, 50 or 100 μM daidzein for 30 min, Total cell lysates were analyzed for pSrc (A) and pERK (B). GAPDH is loading control. *, p ≤ 0.05, **, p ≤ 0.01 vs 0-min group. n = 3.