Figure 3
PSE induced apoptosis, apoptotic body formation, and DNA fragmentation in AGS gastric cancer cells. (A) AGS cells were exposed to 200 μg/ml PSE and 0.1% DMSO for 12 h, 24 h, and 36 h. The DNA content was analyzed using a FACStar flow cytometer, and the percentage of sub-G1 phase cells was determined based on a DNA content histogram. (B) AGS cells were exposed to 200 μg/ml PSE and 0.1% DMSO for 12 h, 24 h, and 36 h. The percentage of morphologically changed cells was determined based on a FSC/SSC dotplot. (C) AGS cells were exposed to 200 μg/ml PSE, 0.1% DMSO and 40 μM cisplatin for 24 h. DNA fragmentation was analyzed using a 1.2% agarose gel for electrophoresis. (D) AGS cells were exposed to 200 μg/ml PSE, 0.1% DMSO and 40 μM cisplatin for 24 h. The morphology of cells was imaged using light microscopy (x200). The data shown are representative of three independent experiments that gave similar results. Bars, SD. *p < 0.05, **p < 0.01, ***p < 0.001, control versus PSE-treated cells.