PSE does not induce the intrinsic apoptosis pathway in AGS gastric cancer cells. (A) AGS cells were exposed to 200 μg/ml PSE and 0.1% DMSO for 12 h and 24 h. The cell lysates were separated by SDS-PAGE gel electrophoresis and Western blotting with specific antibodies was performed (anti-procaspase-9, anti-cleaved caspase-9, anti-cleaved-Bax, anti-cleaved-Bcl-2, and anti-α-actin). (B) The quantification of the Bcl-2/Bax ratio of the band density was performed. (C) AGS cells were treated with 200 μg/ml PSE and 0.1% DMSO for 24 h, washed twice with PBS and stained with 4 μg/ml JC-1. The data shown are representative of three independent experiments that gave similar results.