Mapping domains on MALT1 required for inducing the cleavage of BCL10GFP. (A) Schematic diagram of the deduced protein domains in human MALT1 and deletion mutants. The numbers indicate the start and the end of the domains. The predicted catalytic residues (His 415, Cys464) of the caspase-like domain were marked. (B) (C) Western blot analysis of lysates from cells transfected with various constructs as indicated using anti-BCL10, anti-MALT1, anti-GFP antibodies. Arrow head indicated the expected expression product of each MALT1 expression vector. Mouse anti-MALT1 antibody (sc-46677, Santa Cruz) was raised against amino acids 525-824 of MALT1. The poor signal in lanes 4 and 5 (panel B) was due to the inferior recognition by this anti-MALT1 antibody. B: BCL10; BG: BCL10GFP; P-BG: Phosphorylated BCL10GFP.