Expression of KCNQ1OT1, CDKN1C, H19, and PLAGL1 and the methylation patterns at the KvDMR1 and H19/IGF2 imprinting control regions is conserved between human and bovine

Table 2 PCR primers and conditions used determine imprinted gene expression and DNA methylation

Gene/ICR Symbol	Primers (5′-3′)		PCR Annealiang Tm (°C)	PCR size (bp)	Primer [] μM	MgCI₂(9mM)	#Cycles
H19	Forward	GATATGGTCCGGTGTGATGGAGAGAGCA	62.8	752	0.3	2.5	35
H19	Reverse	TTCGGAGCCTCCAGACTGCGGTG	62.8	752	0.3	2.5	35
KCNQ1OT1	Forward	TCGAGGGTACCGGATTCCCAGGC	64	502	0.3	2.5	35
KCNQ1OT1	Reverse	CGCAGGACACCCCAACTACAGCC	64	502	0.3	2.5	35
CDKN1C	Forward	GGAGGCGCCGCGATCAAGAAG	62	745	0.3	4	35
CDKN1C	Reverse	GACAGCGAAAGCGCGAAGAGAC	62	745	0.3	4	35
PLAGL1	Forward	TCAACCGGAAAGACCACCTGAAGA	60	834	0.3	4	35
PLAGL1	Reverse	GGTCAAAGCCTGCATTGAGCTTGT	60	834	0.3	4	35
H19/IGF2ICR	Forward	GGGGAGGTTGTCGGGTTTATGG	60	493	0.3	2.5	40
H19/IGF2ICR	Reverse	CCGCACCCCTCCTTTAACATC	60	493	0.3	2.5	40
KvDMR1	Forward	TGAGGAGTGAGTTATGAGGA (taurus) TGAGGATTGTAGTTGTGAGGA (indicus)	59.2	419/422	0.3	4	45
KvDMR1	Reverse	CTACCACATCTACCCCAATC	59.2	419/422	0.3	4	45
CDKN1C DMR	Forward	GAGGACTGGGCGTTCCACAGGCCA	62	1108	0.4	GC Buffer II Takara	35
CDKN1C DMR	Reverse	GCCCTTTAACGGCCAGGAGGC	62	1108	0.4	GC Buffer II Takara	35

ISSN: 1423-0127