Analyses of lung fluid and coagulant abnormalities. Sprague Dawley rats were treated with 0.25 mg/kg protective antigen (PA) or 0.25 mg/kg lethal toxin (LT) for 4 h to induce pathogenesis. The experimental outline is illustrated in panel A. (B) Relative levels of albumin (Alb), immunoglobulin (IgG), and fibrinogen (Fib) in rat plasma and lung homogenate tissue fluid were determined. Protein levels in plasma of the vehicle control group were adjusted to 100% in the respective groups (Alb: 3.24 ± 0.26 g/dL, IgG: 797 ± 86 mg/dL, Fib: 119 ± 8 mg/dL; B, vehicle groups, n = 5; ** p < 0.01 compared to lung tissue fluid of both vehicle and PA groups). (C) Relative plasma D-dimer levels were also determined (n = 5; * p < 0.05, LT-4hr vs. PA-4 h groups). (B-C) Data were obtained from two experiments with 2 and 3 replicates, respectively.