Figure 2

Co-administration of curdlan with OVA influences the cytokine expression profiles in draining LNs. Groups of BALB/c mice were sensitized by patch application with OVA plus curdlan or OVA alone. Draining LNs were obtained at 24, 48, 72 and 96 hours after sensitization. Total RNA extraction, cDNA preparation and quantitative real-time PCR for various cytokines were performed. The relative cytokine mRNA expression of each group was normalized to its β-actin expression. Results are shown as means and standard deviations of pooled data from five independent experiments. P values was calculated by Wilcoxon Rank Sum test.