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Table 2 Epitope mapping of Aβ1-42 monoclonal antibody (MAb) 11E12 and polyclonal antisera based on their immunoreactivity with overlapping peptides spanning Aβ1-42 sequence

From: Immunogenic properties of amyloid beta oligomers

Peptide No. aa sequence The reactivity of peptides with different antibodies in ELISA:
MAb 11E12 Antisera to broad size range oligomers Antisera to 1–2 nm oligomers Antisera to 5–10 nm oligomers
1 (aa 1–13) DAEFRHDSGYEVH ++ + ++ +
2 (aa 7–19) DSGYEVHHQKLVF - - + -
3 (aa 13–25) HHQKLVFFAEDVG - - - -
4 (aa 19–31) FFAEDVGSNKGAI - - - -
5 (aa 25–37) GSNKGAIIGLMVG - - - -
6 (aa 31–42) IIGLMVGGVVIA - - - -
  1. Every next peptide was starting with the same 7 aa from the previous one. The last peptide was 12 aa in length. The interaction between peptides and antibodies in an indirect ELISA is expressed as “++” – strong (OD > 1.0), “+” – weak (1.0 > OD > 0.25) and “-“– none (OD < 0.25).