Immunogenic properties of amyloid beta oligomers

Table 2 Epitope mapping of Aβ1-42 monoclonal antibody (MAb) 11E12 and polyclonal antisera based on their immunoreactivity with overlapping peptides spanning Aβ1-42 sequence

Peptide No.	aa sequence	The reactivity of peptides with different antibodies in ELISA:
Peptide No.	aa sequence	MAb 11E12	Antisera to broad size range oligomers	Antisera to 1–2 nm oligomers	Antisera to 5–10 nm oligomers
1 (aa 1–13)	DAEFRHDSGYEVH	++	+	++	+
2 (aa 7–19)	DSGYEVHHQKLVF	-	-	+	-
3 (aa 13–25)	HHQKLVFFAEDVG	-	-	-	-
4 (aa 19–31)	FFAEDVGSNKGAI	-	-	-	-
5 (aa 25–37)	GSNKGAIIGLMVG	-	-	-	-
6 (aa 31–42)	IIGLMVGGVVIA	-	-	-	-

Every next peptide was starting with the same 7 aa from the previous one. The last peptide was 12 aa in length. The interaction between peptides and antibodies in an indirect ELISA is expressed as “++” – strong (OD > 1.0), “+” – weak (1.0 > OD > 0.25) and “-“– none (OD < 0.25).

ISSN: 1423-0127