Figure 5

The surface accessibility is increased in sap6 -null strains. (A) More proteins can be extracted by β-ME from Δsap6 and Δsap9 strains. The plasma membrane protein (Pma1p), ER-chaperone protein (Kar2p), protein transport-related protein (Arf1p), cytoskeleton protein (actin, Act1), and mitochondrial outer membrane protein (porin 1) were detected by specific polyclonal antibodies. (B) Immunofluorescence staining was applied to detect the actin without treating Candida cells into spheroplast. Strains with sap6-deletion (Δsap6, Δsap4/6, and Δsap5/6) and Δsap9 mutant were more accessible to interact with Alexa Fluor™ 488 labeled anti-Act1 antibody. Anti-Mann: anti-mannan antibody.