Figure 8

Expression of pro- and antiapoptotic genes in U937 cells treated with PTX, MG132 and PTX + MG132. U937 human leukemia cells were incubated alone or treated with 8 mM PTX, 1 μM MG132, or PTX + MG132. After 3 hours gene expression was assessed by quantitative Real-Time PCR. The data are expressed as messenger (mRNA) fold change in relative normalized quantities employing the RPL32 gene expression. In all cases, standard deviation was not >0.08. Arbitrary was considered as significant upregulation or downregulation when the change was ≥ 30% in relation to constitutive gene expression.