Figure 3

STIM1-mediated Ca²⁺influx regulates cell migration through focal adhesion turnover and actomyosin contractility. The dynamic interactions among cytoskeleton, non-muscle myosin II and cell-substrate adhesion regulate cell migration. The interaction between ER Ca²⁺ sensor STIM1 and plasma membrane SOC channel Orai1 induces Ca²⁺ influx, which integrates the dynamic interactions between actomyosin and focal adhesion (FA) to mediate efficient cell migration. STIM1/Orai1-mediated Ca²⁺ influx regulates actomyosin formation through the Ca²⁺-dependent myosin light chain kinase (MLCK), accelerates FA turnover through the small GTPase Rac1 and the Ca²⁺-dependent proline-rich tyrosine kinase 2 (Pyk2), and promotes calpain-mediated disassembly and cleavage of FA proteins.