Table 1 Putative differentially recognized polypeptides by the Sm1 serum determined by mass spectrometry
Spot number | Protein name/gene name | Accession number | Measured MW/pI | Theoretical MW/pI | Mascot score (PMF/MS/MS)* | Arginine methylation sites (predicted or experimental) |
|---|---|---|---|---|---|---|
1 | Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial, OXCT1 | P55809 | 53/7.8 | 56578/7.14 | 64/173 | None |
Aldehyde dehydrogenase X, mitochondrial, ALDH1B1 | P30837; B2R8F0; Q8WX76; Q9BV45 | 57626/6.36 | 88/34 | None | ||
2 | Alpha-enolase, ENO1 | GMP2; Q71V37; Q7Z3V6; Q8WU71; Q9UCH6; Q9UM55 | 40/7.7 | 47481/7.01 | x /97 | None |
Splicing factor, proline- and glutamine-rich, SFPQ | P23246; P30808; Q5SZ71 | 76216/4.95 | x/52 | RFR SR GGGGGFHRR G GGGGR GG (7, 9, 19, 25;) [19]; EEER RRRE (571); R GMGPGTPAGYGR G (681,693) [20] | ||
3 | Putative beta-actin-like protein 3, POTEKP | Q9BYX7; Q562N5 | 40/8.1 | 41989/5.91 | x/95 | None |
Alpha-enolase, ENO1 | 47481/7.01 | x/84 | None | |||
4 | Alpha-enolase, ENO1 | 39/8.3 | 47481/7.01 | 99/202 | None | |
5 | LIM and SH3 domain protein 1, LASP1 | Q14847; Q96ED2; Q96IG0 | 33/7.8 | 30097/6.61 | (50)/149 | None |
6 | Heterogeneous nuclear ribonucleoprotein D-like, HNRNPDL | O14979; Q6SPF2; Q7KZ74; Q7KZ75; Q96IM0; Q96S43 | 34/8.3 | 46580/9.59 | none/55 | STYGKASR GG GNHQ (408) [19] |
7 | Cellular nucleic acid-binding protein, CNBP | Q5U0E9; Q6PJI7; Q96NV3 | 19/7.9 | 20704/8.00 | x/56 | TGGGRGRGMR SRGRGGFTSD RGFQFVSSSL [20] |