Immunohistochemical staining of the tumor tissue. (A) Negative EGFRvIII expression is shown on the left, grade 1 in the middle, and grade 2–3 in the right panels. The significant cytoplasmic staining is shown at a higher resolution in the inset square. (B) Representative EGFRwt, PTEN, and pAKT staining results are shown in the left, middle, and right columns, respectively. Examples of negative and positive samples are shown in the upper and lower rows, respectively. A higher resolution of EGFRwt membranous staining is shown in the inset square. Each digital image was directly captured to the computer using light microscopy at a resolution of 200 ×.