Figure 8
Elk1 activation by OME and pCPT in human prostate tissue. Samples from one prostate were incubated for 2 h with OME (30 μM), pCPT (30 μM), or water (solvent), and subsequently analyzed by an Elk1-specific EMSA. Binding of Elk1 to the specific, biotin-labelled DNA probe causes a shift in migration during electrophoresis, as indicated in (A). Experimental conditions were approved by preparation of a negative control using an unlabelled probe, which was added to a sample besides the labelled probe, resulting in competition and disappearence of bands. Shown is a representative experiment in (A), and densitometric quantification in (B) from experiments with prostates from n=5 patients (* p<0.04 vs. H2O).