Figure 3

The methylation status of NDRG1 gene in the fragment from −379 to +86. (A) The CpG island of NDRG1 surrounding the transcription start site. An fragment of 465 bp (−379 to +86), encompassing 49 CpG dinucleotides with the CpG island was selected for methylation analysis. (B) The bisulfite genomic sequencing of eight individual clones was analyze in MDA-MB-231 (MDA) and T47D breast cancer cell lines. The forty-nine dinucleotides are numbered in agreement with the sequence. The open circles represent the unmethylated dinucleotides while the gray to blank portion represents the percentage of methylation. On the above side methylation pattern are represented according to data of the methylation percentage value. The arrows below the CpG dinucleotides represent the MSP primers that were used.