Figure 3

Effect of Cudrania cochinchinensis on nitric oxide production induced by IFN-γ, fAβ and LPS in BV-2 cells. BV-2 cells were treated with indicated concentration of CC-EW (a, b, e, f), 20 μM of CCA to CCF (c, d) or indicated concentration of CCB for 2 h, and then treated with 0.2 ng/ml IFN-γ alone, IFN-γ combined with 1 μM fAβ or 10 ng/ml LPS for 24 h. (a, c, e) After treatment, the cultured medium was collected and the nitrite content was determined. (b, d, f) The cell viability of the treated cells was assayed by MTT reduction. Results are means ± S.D. from three independent experiments. Significant differences between the cells treated with vehicle (Veh) and CC-EW or the compound, CCA to CCF, are indicated by ***, p < 0.001.