Figure 4

Effect of Cudrania cochinchinensis on iNOS expression induced by IFN-γ combined with fAβ in BV-2 cells. BV-2 cell were treated with 0.2 ng/ml IFN-γ (I), 1 μM fAβ (A), IFN-γ combined with fAβ (IA). Alternatively, BV-2 cell were treated with 20 μg/ml CC-EW (IAC) or 20 μM CCB (IACB) for 2 h, and then were treated with IFN-γ combined with fAβ for 24 h. Cell treated with 10 ng/ml LPS (L) was used as positive control. (a) Cells were fixed, subjected to iNOS staining by anti-iNOS antibody (green) and nucleus staining using Hoechst 33258 (blue). (b) The levels of iNOS positive cells (%) are total count from three different fields. (c) The level of iNOS in cell lysate was determined by immunoblotting using β-actin as internal standard. (d) The relative level of iNOS in cell lysate exhibited as percentage of that in IA. Results are means ± S.D. from three independent experiments. Significant differences between the IA and the other treatments are indicated by ***, p < 0.001.