Figure 5

Morphological alteration of the microglia stimulated by LPS, IFN-γ, oAβ alone or combined with IFN-γ. Glial cells treated with vehicle (Veh), 10 ng/ml LPS, 0.5 ng/ml IFN-γ (IFN), or 1–10 μM oAβ (Aβ1, Aβ5, Aβ10) for 24 h. (a) Cells were fixed and subjected to immuno-staining of microglia by anti-Iba1 antibody (red), astrocyte by anti-GFAP antibody (green) and nuclei staining using Hoechst 33258 (blue). Results are repeated for three times, and represent photographs are shown. (b) Form factor of microglial morphology were calculated as describe in the Method section. Results are means ± S.D. from three independent experiments and total nine cells of each group were analyzed. Significant differences between the cells treated with vehicle and the other treatments are indicated by *, p < 0.05, ***, p < 0.001.