Figure 1

CD8⁺T cells are significantly increased in CIN 1 patients. Flow cytometric analysis of peripheral blood in CIN 1 patients or normal donors was performed in order to evaluate variations in the percentages of T cells and NK cells. Gate was drawn around the lymphocyte population, which was then analyzed separately with the corresponding antibodies (CD3⁺ FITC/CD4⁺ PECy5, CD3⁺ FITC/CD8⁺ PECy5, and CD3^- FITC/CD56⁺ PECy5 for detection of CD4 T cells, CD8 T cells and NK cells, respectively). Analysis from the lymphocyte gate shows that both CD4⁺ T cells and CD8⁺ T cells were increased in CIN 1 patients, while NK cells were diminished; however, we only observed a significant increase in the CD8⁺ T cell population. Statistical analysis was performed through Mann–Whitney U test and data were expressed as mean ± SEM; *p = 0.015.