Autophagic flux is induced in the brain tissue of DV2-infected mice. The six-day-old ICR suckling mice were pre-treated with 3-MA (80μg/g) or PBS by intracranial inoculation followed by DV2 (2.5 x 105 pfu/mouse) inoculation 2 h later. The brain tissues of two mice from each group were harvested and total protein lysate was collected at day 3 and day 5 p.i. and analyzed by Western blotting to measure the expression levels of LC3I, LC3II, p62, and DV2-NS1. The numbers under each band are the quantification and the mean of the intensity of LC3II, p62, and NS1 bands after normalization with β-actin.